abstract：:A quantitative assay using a reverse transcriptase-linked polymerase chain reaction has been developed for measuring the levels of rANP and iso-rANP mRNA. A linear correlation between total RNA template and amplified cDNA was obtained for the amplification of cDNA from both iso-rANP and rANP mRNAs even when both cDNAs...

journal_title：Molecular and cellular endocrinology

authors： Roy RN,Pang SC,Flynn TG

更新日期：1992-07-01 00:00:00

abstract：:Renal hypertrophy and hyperfiltration are early manifestations of human and experimental diabetes that may contribute to the late development of diabetic nephropathy. The biochemical events resulting in kidney growth in the diabetic state are completely unknown. Since growth of various tissues is accompanied by increa...

journal_title：Molecular and cellular endocrinology

authors： Pedersen SB,Flyvbjerg A,Grønbaek H,Richelsen B

更新日期：1992-07-01 00:00:00

abstract：:The phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) is a potent activator of protein kinase C (PKC) and is known to affect a variety of biochemical processes in human breast cancer cells. In the present study we have employed MCF-7 cells to investigate the effects of TPA on inositol lipid signalling, the puta...

journal_title：Molecular and cellular endocrinology

authors： Patel KV,Schrey MP

更新日期：1992-06-01 00:00:00

abstract：:The present study examines the involvement of cAMP in the transduction of the short-term effect of gonadotropin-releasing hormone (GnRH) on gonadotropin release in the teleost fish, tilapia. A 5 min pulse of dibutyryl cyclic AMP (dbcAMP; 0.03-3 mM) or forskolin (0.1-10 microM) resulted in dose-dependent surges in tila...

journal_title：Molecular and cellular endocrinology

authors： Levavi-Sivan B,Yaron Z

更新日期：1992-06-01 00:00:00

abstract：:Evidence that transforming growth factor-beta (TGF beta) is produced by porcine thecal cells and acts upon porcine granulosa cells suggests that this peptide may be a local regulator of follicular function in this species. The objective of the present study was to investigate the effects of TGF beta on steroidogenesis...

journal_title：Molecular and cellular endocrinology

authors： Engelhardt H,Tekpetey FR,Gore-Langton RE,Armstrong DT

更新日期：1992-05-01 00:00:00

abstract：:The regulation of heat shock protein 90 kDa (hsp90) by estradiol was analyzed in the rat ventromedial hypothalamus (VMH) and uterus by one-dimensional gel electrophoresis/Western blots. Protein from VMH and uterus (35 micrograms/sample) was resolved on 8% acrylamide gels, transferred to polyvinyldifluoride filters, an...

journal_title：Molecular and cellular endocrinology

authors： Olazábal UE,Pfaff DW,Mobbs CV

更新日期：1992-04-01 00:00:00

abstract：:Recently, a defect in pertussis toxin-independent actions of epinephrine on pancreatic B-cells of fa/fa Zucker rats was reported (Cawthorn and Chan (1991) Mol. Cell. Endocrinol. 75, 197-204). We now report studies of islet alpha 2-adrenoceptor function of fa/fa rats. Insulin and cAMP production by islets of obese rats...

journal_title：Molecular and cellular endocrinology

authors： Chan CB,MacPhail RM

更新日期：1992-03-01 00:00:00

abstract：:Polymerase chain reaction (PCR) amplification of nt 4502 to nt 5184 of the thyroglobulin (Tg) mRNA from several patients, with or without elevated serum thyrotropin (TSH), showed a predominant fragment of the expected size (683 bp) and a minor fragment of 512 bp. The sequence of this minor fragment revealed that 171 b...

journal_title：Molecular and cellular endocrinology

authors： Targovnik HM,Cochaux P,Corach D,Vassart G

更新日期：1992-03-01 00:00:00

abstract：:Chemotactic and mitogenic activities of granulosa cells in developing follicles were studied. Immature rats were subcutaneously injected with 20 IU of pregnant mare's serum gonadotrophin and killed at various intervals after injection. The ovaries were removed and granulosa cells were isolated and cultured in a serum-...

journal_title：Molecular and cellular endocrinology

authors： Lin MT,Wei SJ,Wing LY

更新日期：1992-03-01 00:00:00

abstract：:Rat ovarian membrane luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor was reconstituted into proteoliposomes. The ability of sodium cholate to extract and reconstitute hCG binding activity was dependent on the protein/detergent ratio. Trypsinization of the LH/hCG receptor containing proteoliposomes i...

journal_title：Molecular and cellular endocrinology

authors： Kolena J,Matejcíková K,Srenkelová G

更新日期：1992-02-01 00:00:00

abstract：:The primary aim of this study was to evaluate the effects of arachidonic acid (AA) on calcium mobilization from intracellular compartments in digitonin-permeabilized granulosa cells isolated from the largest preovulatory follicles of laying hens. At low concentrations (ED50 0.2 microM) AA released 35% 45Ca from the en...

journal_title：Molecular and cellular endocrinology

authors： Hertelendy F,Molnár M,Jamaluddin M

更新日期：1992-02-01 00:00:00

abstract：:Total RNA from several adult (6-18-month-old) rabbit tissues was characterized using an oligonucleotide probe derived from the extracellular domain of the nucleotide sequence of the rabbit growth hormone receptor (GH-R) cDNA. Multiple GH-R mRNA species of approximately 4.6, approximately 3.3, 2.1 and approximately 1.4...

journal_title：Molecular and cellular endocrinology

authors： Ymer SI,Herington AC

更新日期：1992-01-01 00:00:00

abstract：:Platelet-derived growth factor (PDGF) is a potent mitogen for several cell types. In addition, PDGF has vasoconstrictive action and shares some signal transduction mechanisms with angiotensin II (AII). In the present study, we have examined the effects of PDGF on basal and AII-induced aldosterone synthesis in freshly ...

journal_title：Molecular and cellular endocrinology

authors： Natarajan R,Nadler J

更新日期：1992-01-01 00:00:00

abstract：:The age-related evolution of the in vitro effects of a gonadotropin releasing hormone agonist ([D-Trp6]-GnRH) on the secretion of testosterone by the testis, cultured during 3 days on a Millipore filter floating on M199 medium, was studied during the perinatal period in the rat. The basal and luteinizing hormone (LH)-...

journal_title：Molecular and cellular endocrinology

authors： Habert R,Devif I,Gangnerau MN,Lecerf L

更新日期：1991-12-01 00:00:00

abstract：:The mouse mammary tumor virus (MMTV) initiates infection when the envelope protein, gp52, binds to a cell surface protein and triggers internalization. The hormonal regulation of this protein was studied both in vitro and in vivo. In mammary gland explant culture, levels of the MMTV binding protein were maintained onl...

journal_title：Molecular and cellular endocrinology

authors： Bolander FF Jr

更新日期：1991-12-01 00:00:00

abstract：:On the surface of the free (receptor-unbound) form of hCG, we have previously identified 14 topographically distinct epitopes (Schwarz et al. (1986) Endocrinology 118, 189-197; Berger et al. (1990) J. Endocrinol. 125, 301-309). Here we report that only two of them, i.e. the (adjacent) beta 3 and beta 5 epitopes, can b...

journal_title：Molecular and cellular endocrinology

authors： Schwarz S,Krude H,Wick G,Berger P

更新日期：1991-11-01 00:00:00

abstract：:To elucidate the cellular origin of interleukin-1 (IL-1) in the mammalian testis, we assayed IL-1 activity in culture media of Sertoli cells collected from rats at 20, 35 and 45 days of age as well as in culture media of interstitial, peritubular and germ cells from adult rats. IL-1 was detected in Sertoli cell cultur...

journal_title：Molecular and cellular endocrinology

authors： Gérard N,Syed V,Bardin W,Genetet N,Jégou B

更新日期：1991-11-01 00:00:00

abstract：:The ability of angiotensin-II (A-II) to increase cAMP production in adrenocortical cells is not widely accepted due to numerous conflicting reports. The recent observation that rat adrenal cells exhibit multiple subtypes of A-II receptors raises the possibility that a specific subtype could be responsible for controll...

journal_title：Molecular and cellular endocrinology

authors： Rainey WE,Byrd EW,Sinnokrot RA,Carr BR

更新日期：1991-10-01 00:00:00

abstract：:The effects of angiotensin II on cytosolic free Ca2+ ion concentrations ([Ca2+]i) were studied in single porcine granulosa cells using the calcium-sensitive fluorescent dye fura-2 and high temporal resolution fluorescent videomicroscopy. Angiotensin II initiated specific, rapid, transient and topographically organized...

journal_title：Molecular and cellular endocrinology

authors： Flores JA,Veldhuis JD,Leong DA

更新日期：1991-10-01 00:00:00

abstract：:Within the seminiferous tubules, the Sertoli cells create an impermeable blood-testis barrier and an unique intratubular microenvironment that fosters the development of spermatozoa. The functional differentiation of spermatozoa therefore requires vectorial secretion by Sertoli cells of substances that cannot cross th...

journal_title：Molecular and cellular endocrinology

authors： Spaliviero JA,Handelsman DJ

更新日期：1991-10-01 00:00:00

abstract：:The detergent-soluble extract of rat ovary plasma membranes contained a Gs protein of about 100 kDa as shown by its elution behavior on a Bio Gel A-1.5m column. However, the cell membranes exposed to hCG (37 degrees C, 15 min) contained in addition a higher molecular weight Gs protein complex of 300 kDa comprised of h...

journal_title：Molecular and cellular endocrinology

authors： Seth PK,Bahl OP

更新日期：1991-09-01 00:00:00

abstract：:Secretogranin I (SgI; chromogranin B) belongs to a class of acidic tyrosine-sulfated secretory proteins believed to play a role in the secretory process of endocrine cells. Our aim here was to compare the levels of SgI mRNA to that of prolactin (PRL) and growth hormone (GH), using rat pituitary cell lines. As far as t...

journal_title：Molecular and cellular endocrinology

authors： Laverrière JN,Richard JL,Morin A,Buisson N,Tixier-Vidal A,Huttner WB,Gourdji D

更新日期：1991-09-01 00:00:00

abstract：:The relationship between ATP-induced uptake of 45Ca2+ and the ATP-induced changes in [Ca2+]i was investigated in rat FRTL-5 thyroid cells. Addition of 1 microCi 45Ca2+/ml together with ATP induced a time- and dose-dependent increase in uptake of 45Ca2+, the uptake being still significantly above control after 30 min. ...

journal_title：Molecular and cellular endocrinology

authors： Törnquist K

更新日期：1991-08-01 00:00:00

abstract：:Direct roles of follicle-stimulating hormone (FSH)-suppressing protein (FSP) and activin in regulation of ovarian granulosa cell differentiation have been reported recently. The present study further investigated the effects of these peptides on steroidogenesis and inhibin production as well as cAMP generation in cult...

journal_title：Molecular and cellular endocrinology

authors： Xiao S,Findlay JK

更新日期：1991-08-01 00:00:00

abstract：:We report a novel method to study the mechanisms of luteinizing hormone (LH) receptor desensitization and truncation, using antisense oligodeoxynucleotides that code for regions of the NH2-terminus, the third extracellular loop and the C-terminus of the LH receptor. Mouse tumour (MA10) Leydig cells were incubated for ...

journal_title：Molecular and cellular endocrinology

authors： West AP,Cooke BA

更新日期：1991-08-01 00:00:00

abstract：:Specific binding sites for bovine placental lactogen (bPL) and the lactogenic hormone, prolactin, have been detected in endometrial membranes isolated from uteri of mid-pregnant heifers. The specific binding of human growth hormone (hGH) (used to monitor the presence of lactogenic binding sites) and of bPL was increas...

journal_title：Molecular and cellular endocrinology

authors： Galosy SS,Gertler A,Elberg G,Laird DM

更新日期：1991-07-01 00:00:00

abstract：:We have localized four transcription initiation sites in the human insulin-like growth factor-I (IGF-I) gene. Two transcription start sites were identified which result in a longer and shorter version of the leader derived from the known exon 1 of the IGF-I gene. Transcription starting at the upstream transcription in...

journal_title：Molecular and cellular endocrinology

authors： Jansen E,Steenbergh PH,LeRoith D,Roberts CT Jr,Sussenbach JS

更新日期：1991-06-01 00:00:00

abstract：:In the 40 years of transferrin research, no previous role for apotransferrin has been recognized other than to serve as a plasma carrier for dietary and storage iron. Our studies have revealed a new 'autocrine' growth role for this molecule as well as a possible new cell-cell bridge/CAM function. Certainly, these obse...

journal_title：Molecular and cellular endocrinology

authors： Sirbasku DA,Pakala R,Sato H,Eby JE

更新日期：1991-05-01 00:00:00

abstract：:We have shown previously that activin A increases the number of immunoreactive follicle-stimulating hormone (FSH) cells. To further investigate the action of activin A, we examined its effects on anterior pituitary cells fractionated by centrifugal elutriation. Before activin A treatment, FSH cells were widely distrib...

journal_title：Molecular and cellular endocrinology

authors： Katayama T,Shiota K,Takahashi M

更新日期：1991-05-01 00:00:00

abstract：:We have designed and synthesized a biotinylated vasopressin antagonist which is a selective probe for studying the V1a subtype of vasopressin receptor. Initially we synthesized the novel vasopressin analogue d(CH2)5Tyr(Me)2LysNH2(9)AVP (ALVP). Biotinamidocaproate was subsequently coupled to the epsilon-amino group of ...

journal_title：Molecular and cellular endocrinology

authors： Howl J,Kerr ID,Chan CH,Wheatley M

更新日期：1991-05-01 00:00:00

abstract：:The screening of an oligo(dT)-primed prostate cDNA library with a human glandular kallikrein-1 (hGK-1) genomic DNA fragment resulted in the isolation of two different hGK-1 cDNAs. A 1.2 kb cDNA (pGK-1) contains an open reading frame of 510 bp, encoding the major part of the previously predicted hGK-1 protein (Schedlic...

journal_title：Molecular and cellular endocrinology

authors： Riegman PH,Vlietstra RJ,van der Korput HA,Romijn JC,Trapman J

更新日期：1991-04-01 00:00:00

abstract：:We have previously shown that osteocalcin synthesis is readily induced by 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) in MG-63 human osteosarcoma cells (Mahonen et al. (1990) Biochim. Biophys. Acta 1048, 30-37). In the present study, the regulation of osteocalcin synthesis by other hormones of the steroid-thyroid hormone f...

journal_title：Molecular and cellular endocrinology

authors： Pirskanen A,Mahonen A,Mäenpää PH

更新日期：1991-04-01 00:00:00

abstract：:Porcine Leydig cells in primary culture were incubated to equilibrium with increasing doses of either porcine luteinizing hormone (pLH) or human chorionic gonadotropin (hCG). Then, free and total high affinity gonadotropin receptors on the cell surface were dosed with [125I]hCG as tracer. In isotonic high salt medium,...

journal_title：Molecular and cellular endocrinology

authors： Genty N,Salesse R,Jallal B,Garnier J

更新日期：1991-04-01 00:00:00

abstract：:The conversion of 3 beta-hydroxy-5-ene steroids by the enzyme complex 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase (3 beta-HSD) is an obligatory step in the biosynthesis of all classes of hormonal steroids in classical steroidogenic as well as in peripheral tissues. To develop a model more closely rel...

journal_title：Molecular and cellular endocrinology

authors： Simard J,Melner MH,Breton N,Low KG,Zhao HF,Periman LM,Labrie F

更新日期：1991-02-01 00:00:00

abstract：:We have studied a 15-year-old girl (P1) suffering from the Hutchinson-Gilford syndrome (progeria) associated with a severe insulin resistance. Insulin binding activity to P1 erythrocytes was 85% reduced when compared to that measured in ten normal controls matched for sex and age. This finding was confirmed in Epstein...

journal_title：Molecular and cellular endocrinology

authors： Briata P,Bellini C,Vignolo M,Gherzi R

更新日期：1991-01-01 00:00:00

abstract：:The ovarian granulosa cell has previously been shown to be a site of insulin-like growth factor (IGF) I production, reception, and action. It is the objective of this study to characterize in greater detail the soluble IGF binding activity released by this cell type. To this end, use was made of granulosa cells from i...

journal_title：Molecular and cellular endocrinology

authors： Adashi EY,Resnick CE,Hernandez ER,Hurwitz A,Rosenfeld RG

更新日期：1990-12-21 00:00:00

abstract：:Four abundant cDNA clones have been isolated from a rat epididymal cDNA library. Northern blot analysis has shown that these clones partially encode 4.5 kb, 2.8 kb, 1.2 kb and 0.85 kb mRNAs and that their expression is not detectable in total RNA preparations from heart, kidney, liver or testis. Fourteen days after ca...

journal_title：Molecular and cellular endocrinology

authors： Walker JE,Jones R,Moore A,Hamilton DW,Hall L

更新日期：1990-11-12 00:00:00

abstract：:Thymocytes from SJL/J mice exhibit a periodicity in their response to hormonal stimulation with isoproterenol. This periodicity (5-9 days) is expressed in large changes in the intensity of the response (peak levels of intracellular cAMP which vary approximately 6-fold), and in the response pattern, i.e., in the occurr...

journal_title：Molecular and cellular endocrinology

authors： Riven-Kreitman R,Tauber-Finkelstein M,Zipori D,Shaltiel S

更新日期：1990-10-22 00:00:00

abstract：:Neonatal and adult rat islets, cultured for 7-9 days in the presence of 10.5 mM D-glucose, were incubated for 120 min with either D-glucose (2.8 and 16.7 mM) or L-leucine (1.0 and 20.0 mM). The total and anaerobic rates of glycolysis, as judged respectively through the generation of 3H2O from D-[5-3H]glucose and 14C-l...

journal_title：Molecular and cellular endocrinology

authors： Boschero AC,Bordin S,Sener A,Malaisse WJ

更新日期：1990-10-01 00:00:00

abstract：:The degradation products generated from A14 and B26 125I-labelled insulins in liver endosomes in vivo and in vitro have been isolated by high-performance liquid chromatography and cleavages in the B chain have been identified by automated radiosequence analysis. In rats sacrificed various times after injection of each...

journal_title：Molecular and cellular endocrinology

authors： Clot JP,Janicot M,Fouque F,Desbuquois B,Haumont PY,Lederer F

更新日期：1990-09-10 00:00:00